The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes
Identifieur interne : 003807 ( Main/Exploration ); précédent : 003806; suivant : 003808The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes
Auteurs : Leiv Ose [Norvège] ; Turid Ose [Norvège] ; Kaare R. Norum [Norvège] ; Trond Berg [Norvège]Source :
- Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism [ 0005-2760 ] ; 1980.
English descriptors
- Teeft :
- Acid phosphatase, Acta, Apolipoprotein, Beckman ultracentrifuge, Berg, Biol, Biophys, Bovine serum albumin, Centrifugation, Centrifuge tube, Chloroquine, Differential centrifugation, Enzyme activities, Hepatocytes, High density lipoproteins, Homogenate, Incubation, Incubation medium, Intracellular, Isopycnic, Isopycnic centrifugation, Light mitochondrial, Linear sucrose gradients, Lipoprotein, Lysosome, Major part, Marker enzymes, Mitochondrial, Phosphatase, Plasma cell membrane, Postnuclear fractions, Radioactivity, Speed centrifugation, Subcellular, Subcellular distribution, Subcellular fractions, Sucrose, Sucrose gradients, Sucrose solution, Unlabelled, Vivo.
Abstract
Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.
Url:
DOI: 10.1016/0005-2760(80)90191-5
Affiliations:
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Norum</name></author><author><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:28E7CF38E2C413AF75BB37C40E8A0CA410535E96</idno><date when="1980" year="1980">1980</date><idno type="doi">10.1016/0005-2760(80)90191-5</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-RGH25CV5-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C17</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C17</idno><idno type="wicri:Area/Istex/Curation">000C17</idno><idno type="wicri:Area/Istex/Checkpoint">002563</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002563</idno><idno type="wicri:doubleKey">0005-2760:1980:Ose L:the:intracellular:distribution</idno><idno type="wicri:Area/Main/Merge">003886</idno><idno type="wicri:Area/Main/Curation">003807</idno><idno type="wicri:Area/Main/Exploration">003807</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">The intracellular distribution of high density lipoproteins taken up by isolated rat hepatocytes</title><author><name sortKey="Ose, Leiv" sort="Ose, Leiv" uniqKey="Ose L" first="Leiv" last="Ose">Leiv Ose</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Ose, Turid" sort="Ose, Turid" uniqKey="Ose T" first="Turid" last="Ose">Turid Ose</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Norum, Kaare R" sort="Norum, Kaare R" uniqKey="Norum K" first="Kaare R." last="Norum">Kaare R. Norum</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name><affiliation wicri:level="1"><country xml:lang="fr">Norvège</country><wicri:regionArea>Institute for Nutrition Research, School of Medicine, University of Oslo, Blindern, P.O. Box 1046, Oslo 3</wicri:regionArea><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism</title><title level="j" type="abbrev">BBALIP</title><idno type="ISSN">0005-2760</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1980">1980</date><biblScope unit="volume">620</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="120">120</biblScope><biblScope unit="page" to="132">132</biblScope></imprint><idno type="ISSN">0005-2760</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0005-2760</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acid phosphatase</term><term>Acta</term><term>Apolipoprotein</term><term>Beckman ultracentrifuge</term><term>Berg</term><term>Biol</term><term>Biophys</term><term>Bovine serum albumin</term><term>Centrifugation</term><term>Centrifuge tube</term><term>Chloroquine</term><term>Differential centrifugation</term><term>Enzyme activities</term><term>Hepatocytes</term><term>High density lipoproteins</term><term>Homogenate</term><term>Incubation</term><term>Incubation medium</term><term>Intracellular</term><term>Isopycnic</term><term>Isopycnic centrifugation</term><term>Light mitochondrial</term><term>Linear sucrose gradients</term><term>Lipoprotein</term><term>Lysosome</term><term>Major part</term><term>Marker enzymes</term><term>Mitochondrial</term><term>Phosphatase</term><term>Plasma cell membrane</term><term>Postnuclear fractions</term><term>Radioactivity</term><term>Speed centrifugation</term><term>Subcellular</term><term>Subcellular distribution</term><term>Subcellular fractions</term><term>Sucrose</term><term>Sucrose gradients</term><term>Sucrose solution</term><term>Unlabelled</term><term>Vivo</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The subcellular distribution of 125I-labelled HDL taken up by rat hepatocytes in vivo and in vitro has been studied with subcellular fractionation techniques: differential centrifugation and isopycnic centrifugation in sucrose gradients. 125I-labelled HDL bind to plasma membranes both in vivo and in vitro and part of the membrane-bound 125I-labelled HDL can be dissociated by the addition of unlabelled HDL. The hepatocytes also internalize 125I-labelled HDL. The 125I-labelled HDL accumulate, however, at different intracellular sites in the in vivo and in vitro situation. The subcellular distribution pattern of 125I-labelled HDL taken up by the cells in vivo is similar to that of the lysosomal marker enzyme acid phosphatase. Peak activity was found at a density of 1.20 g/ml. In vitro 125I-labelled HDL accumulate in an organelle with a medium density of about 1.13 g/ml. This distribution was similar to that of the plasma membrane marker 5'-nucleotidase. The subcellular distribution of radioactivity taken up in vivo was changed to lower density by incubating the cells with chloroquine, a drug known to render the lysosomes more boyant. Chloroquine had no effect on the distribution of 125I-labelled HDL taken up by hepatocytes in vitro.</div></front></TEI><affiliations><list><country><li>Norvège</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><country name="Norvège"><region name="Østlandet"><name sortKey="Ose, Leiv" sort="Ose, Leiv" uniqKey="Ose L" first="Leiv" last="Ose">Leiv Ose</name></region><name sortKey="Berg, Trond" sort="Berg, Trond" uniqKey="Berg T" first="Trond" last="Berg">Trond Berg</name><name sortKey="Norum, Kaare R" sort="Norum, Kaare R" uniqKey="Norum K" first="Kaare R." last="Norum">Kaare R. Norum</name><name sortKey="Ose, Turid" sort="Ose, Turid" uniqKey="Ose T" first="Turid" last="Ose">Turid Ose</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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